FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33342407
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA118900.022.8224478
ACTACCC129900.021.1817176
CTACCCT137350.019.9072517
CTAGTTG143400.019.4281568
TACTCTA152000.019.3721474
CCTACAC151550.018.99787911
ACTCTAG161550.018.6966275
GGACACC147850.017.82040629
TACTACC157800.017.502165
TCTAGTT171700.017.4106737
ACCTCTA163300.017.0391117
CTCTAGT177800.016.677626
GACACCT161400.016.51742630
CTAATGC168050.016.37286221
GTACTCT180850.016.2818663
GCGGGGG30600.016.0077781
CTACACT171950.015.8816512
GCTGGAC170750.015.81225726
CTGTTAC180500.015.8049251
TGTACTC185350.015.7381252
Produced by FastQC (version 0.11.2)