FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12211086
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC222290.18203950082736295No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT149890.12274911502547768No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG142330.11655801949146864No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCA141150.11559168447425562No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACTGAGGGGACAGGATGTCCCAGGCGA133490.1093186961421777No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT128130.10492924216568454No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT122770.10053978818919135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG105450.034.4316255
TACTCTA66500.030.7473014
CTAGTTG71100.028.9044598
GCTGACT57600.028.8069671
CTCTAGT72100.028.5987476
ACTACCC74600.027.501686
TACCCTA74750.027.493078
TCTAGTT77700.026.8929257
GTACTCT77000.026.5545963
CCTACAC83350.026.22861911
CTACCCT79100.025.981027
ACCTCTA77700.025.64992717
GTTACCT78950.025.50607114
TAGTTGT81450.025.4855259
TGTACTC80750.025.3645272
GACTCTA66400.025.2891864
CTGTTAC83000.025.1034721
CTAGCAG66050.025.0063828
TTGTACT82900.024.4263481
CTGACTC68750.024.0741732
Produced by FastQC (version 0.11.2)