FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7953830
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT165190.20768610845341176No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA91900.11554182073290477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA44750.029.0475524
CTCTAGT45550.028.4622256
CTAGTTG46700.028.0564928
ACTCTAG47150.027.9348015
TCTAGTT48450.027.8261017
GTACTCT49350.026.6894823
TGTACTC52150.025.191482
GTTACCT51400.024.88728114
TTGTACT53250.024.4226741
ACCTCTA51950.024.29132817
GGACACC50850.023.39833329
TAGTTGT56300.023.2728779
GACACCT51900.022.92726330
CTAATGC55850.022.71853821
CCTCTAA57150.021.96029518
TACCTCT59350.021.61128816
TTACCTC60300.021.32839415
TGGACAC57000.021.2955628
TGTTACC60700.021.24491513
GCTGGAC58350.020.80168326
Produced by FastQC (version 0.11.2)