FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25444364
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT379680.14921968574258723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCTA116400.026.1866994
TACCCTA113250.026.1844988
ACTACCC117900.025.3856966
CTAGTTG120550.025.1995478
CTCTAGT124400.024.6136346
ACTCTAG127550.024.276065
TCTAGTT136550.023.2566787
CCTACAC136300.023.24919711
CTACCCT128850.023.121137
GTACTCT139100.021.8389173
ACCTCTA138200.021.35713217
GTTACCT139100.021.3185214
CTGTTAC143400.021.2890621
TAGTTGT152200.020.2085869
GACACCT135100.020.16691230
GGACACC136900.020.05180229
CTAATGC144050.020.03504221
TGTACTC151400.020.0197662
TACTACC153100.019.6391935
CTACACT152950.019.61359612
Produced by FastQC (version 0.11.2)