FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5533742
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG60520.10936541674693183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTAG42150.028.878315
CTAGTTG32150.028.2075568
TACTCTA32900.027.6695864
TCTAGTT37550.025.8047
CTCTAGT36600.025.7200786
GTACTCT36150.025.0866173
TGTACTC36200.024.7668742
ACTACCC36400.024.5351726
TACCCTA36400.024.156258
TTGTACT37150.024.0515791
CCTACAC39600.023.59807411
CTGTTAC38700.023.3557031
CTACCCT37800.023.3527957
ACCTCTA37900.023.10921517
GTTACCT38900.022.60440414
CTAATGC38400.022.44933121
GGACACC36050.022.38576529
ATGCTGG40450.021.82485424
TAGTTGT42600.021.6932079
TACCTCT40400.021.59403416
Produced by FastQC (version 0.11.2)