FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10167624
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTACAG219430.21581246513443061No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACAGGATACAT201840.19851245482720445No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT152870.15034977689969653No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC144310.14193089752335453No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC129890.12774862642442325No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA123420.12138529119487503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT105650.033.9568671
TACTCTA110200.032.8226744
ACTCTAG112900.032.3432925
TGTACTC114900.031.3915732
GTACTCT117750.030.893973
CTAGTTG117750.030.8637758
CTGTTAC110700.030.413471
TCTAGTT120700.030.395267
CTCTAGT120450.030.2303776
ACTACCC115400.028.9836046
CTACCCT120150.027.8942057
TACCCTA120250.027.72758
ACCTCTA131000.027.16286817
TACTACC123350.027.1153225
GTTACCT131650.027.02888914
TAGTTGT135850.026.8784089
CCTACAC125900.026.62037511
CTAATGC135900.026.03074521
TTACCTC138300.025.95361715
GTTACTA128500.025.9480973
Produced by FastQC (version 0.11.2)