FastQCFastQC Report
Wed 1 Feb 2017
HGHGWBGX2_n01_ech_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHGWBGX2_n01_ech_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25151117
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCC115800.025.6969686
TACCCTA114300.025.6719388
CTAGTTG102200.024.6627678
CCTACAC132650.022.53676211
TACTCTA115100.022.2280484
ACTCTAG118800.022.2034425
TACTACC136100.021.8639185
TCTAGTT124550.021.732087
CTACCCT136550.021.6403587
GGACACC113500.021.17710729
ACCTCTA118550.021.05726617
CTGTTAC146250.020.7320351
CTCTAGT128600.020.4043756
CTACACT147650.020.03691912
GACACCT121300.019.8734330
CTAATGC125100.019.8445821
GTACTCT133500.019.2419683
GTTACCT132800.018.79799514
ATGCTGG132750.018.75405924
TACACTC158150.018.68477213
Produced by FastQC (version 0.11.2)