FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25910751
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG691620.2669239498307093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA121050.036.263212
GATCGGA123400.035.315761
TCGGAAG130350.033.0260283
CGGAAGA130900.032.3783154
ACACGTC121150.030.90876213
ACGTCTG127650.028.73244515
GCACACG134100.028.65274811
CACACGT133450.028.50397112
CGTCTGA130200.028.00870716
CACGTCT133350.027.76657314
AGAGCAC194850.020.8688478
AGCACAC199450.020.0190910
GAACTCC206550.017.60332921
CTGAACT219650.017.09640719
GAAGAGC270600.015.7397666
GAGCACA259550.015.6936759
TGAACTC245800.015.46176820
AACTCCA257550.014.07673122
AAGAGCA311500.013.8065937
TCTGAAC285400.013.4643818
Produced by FastQC (version 0.11.5)