FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24790020
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC754900.3045177051087494TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG111500.058.7658643
CGTATGC121350.057.05282644
CTCGTAT102350.056.8724642
TATGCCG124100.056.12395546
TCTCGTA107050.052.8388341
ATGCCGT130750.051.8547
GTATGCC140050.050.7073845
ATCTCGT119700.049.8864440
CGTCCAT174700.049.42350835
ACCCGTC188800.048.88405632
ACACGTC194650.048.46060613
CGTCTGA195350.048.16318516
ACGTCTG197150.047.77671415
ATCGGAA213600.045.3872762
CCGTCCA202100.045.21650734
CACGTCT209050.045.1739114
CACCCGT210450.044.0047931
GATCGGA221250.043.6511651
CCGTCTT145100.042.95905350
CACACGT221850.042.6268512
Produced by FastQC (version 0.11.5)