FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28576171
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATGC1812120.6341367428127442TruSeq Adapter, Index 15 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG223250.061.5699443
CTCGTAT218100.060.7129742
CGTATGC228900.060.1416344
TATGCCG231200.059.5240346
TCTCGTA226300.058.03354341
ATGCCGT237600.057.9052547
ATCTCGT234100.056.2047840
AATCTCG236900.055.52550539
GTATGCC250150.055.16939545
GTCAATC246400.053.63693636
ACACGTC273800.052.20919813
CGTCTGA275500.051.6346516
ACGTCTG280000.050.90437315
CCGTCTT269600.050.53837250
ATCGGAA292100.049.9161872
TGCCGTC274900.049.6918448
ACATGTC284250.049.49764632
TGTCAAT279150.049.3487935
TCAATCT268450.049.1405437
CACACGT295200.048.6097612
Produced by FastQC (version 0.11.5)