FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27437434
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATGC853130.3109365110454571TruSeq Adapter, Index 14 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG134850.060.47865343
CGTATGC139000.059.72974444
TCTCGTA112250.059.0598541
CTCGTAT113600.059.03585442
ATGCCGT140100.058.5570847
TATGCCG146750.057.00126646
TGCCGTC140350.056.90656348
GCCGTCT137750.055.28733449
CGTCTGA178050.054.67138716
CCGTCTT141100.054.64432550
ATCTCGT126350.053.7156140
ACGTCTG186100.052.34383415
ACACGTC187850.052.17182513
CGTCTTC142150.052.1231651
CATCTCG134500.050.7206839
GTCGTAT31350.049.68453242
CACGTCT202150.048.3952614
GTATGCC178600.047.1501245
TCGCGTA5750.045.65539641
GCACACG218700.044.92085311
Produced by FastQC (version 0.11.5)