FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22741854
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGC658460.2895366402405011TruSeq Adapter, Index 13 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT85500.055.5131942
TCTCGTA85800.055.27841641
TCGTATG90900.054.94901343
CGTATGC91900.054.5788944
ATGCCGT92600.053.557447
TATGCCG94450.052.99067746
AATCTCG90500.052.21352839
TGCCGTC97700.050.43870548
GCCGTCT99500.049.17449649
ATCTCGT96550.049.01434740
CCGTCTT101650.048.40985550
CGTCTGA112400.047.67770416
ACACGTC114900.046.6678213
CGTCTTC104100.046.66525351
GATCGGA119950.045.466431
ATCGGAA121250.045.2027552
ACGTCTG118900.044.9233815
CACGTCT127000.042.22290814
GTATGCC121850.041.47752445
TCGGAAG133250.041.052993
Produced by FastQC (version 0.11.5)