FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16916659
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG21450.021.05021743
CTCGTAT21200.020.63803542
CGTATGC23150.018.89945644
TCTCGTA22950.018.60678341
TATGCCG24700.018.13750846
ATGCCGT27400.016.47782747
ACGGCTA26850.016.16341232
ATCTCGT29950.014.4916940
TGCCGTC33450.013.49753348
CGGCTAC35250.012.90743333
CCGTCTT37150.012.24736850
CATCTCG38250.011.71312239
TCACGGC41100.011.32567730
GCCGTCT40100.011.17184949
CGTCTTC42550.011.10436451
CGTCTGA42750.010.88983316
ATCGGAA42200.010.7816822
ACACGTC42950.010.75718213
GATCGGA43400.010.5657761
AGTCACG44350.010.49575428
Produced by FastQC (version 0.11.5)