FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25069523
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1616060.6446313318366688TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG203750.060.2461443
TCTCGTA196300.060.1977441
CTCGTAT198200.060.06217242
CGTATGC205400.059.64253644
CGATCAG206650.059.5821333
ATGCCGT205900.059.4936747
GTCACGA209350.059.097929
GATCTCG198650.059.0801139
ACGATCA209900.058.8763532
TGCCGTC208250.058.77177448
GATCGGA220900.058.6000251
CACGATC212050.058.27939631
TATGCCG210850.058.26377546
GCCGTCT210500.058.02707349
ATCGGAA224950.057.768962
CCGTCTT211800.057.7370150
CGTCTGA216100.057.7109516
ATCTCGT204450.057.4214340
ACACGTC220850.057.21244413
TCACGAT216800.057.08323730
Produced by FastQC (version 0.11.5)