FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24090511
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA60750.053.184281
ATCGGAA65250.049.937082
TCGCGTA6150.049.51634241
CGTCTGA67850.047.2036616
ATCGCGT9900.047.02404440
ACACGTC69100.046.80420313
ACGTCTG72800.044.18621415
CGCGTAT7400.041.6251142
TCGGAAG78800.041.4389273
AATCGCG16950.040.26869239
TGAATCG30050.039.48553537
CGGAAGA83300.039.11634
AATCTCG37250.037.58688739
TCTCGTA29550.037.4312341
CACGTCT86150.037.37933314
CTCGTAT30200.036.97329742
GAATCGC19150.036.373238
CGTATGC30050.035.99243544
CACACGT92700.034.99937412
GCACACG92550.034.9422111
Produced by FastQC (version 0.11.5)