FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22036431
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC364700.16549866899953083TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG57700.053.32245343
CTCGTAT59200.050.67073442
CGTATGC65800.049.2048644
TATGCCG66500.048.94730846
ATGCCGT69950.045.331547
TCTCGTA66600.044.56764641
ATCTCGT72800.042.55101840
GTATGCC79050.041.929345
TAGGCAT109850.036.9584435
ACACGTC118100.036.187213
CGTCTGA118250.036.0832416
ACGTCTG120150.035.51247815
TTAGGCA123600.033.61142734
TCACTTA127050.033.3876330
ATCGGAA131550.032.9902462
CACTTAG132450.031.99998731
CACGTCT134900.031.88878814
CCGTCTT92800.031.75570550
TGCCGTC97950.031.26520248
CATCTCG102000.031.22750939
Produced by FastQC (version 0.11.5)