FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27595291
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC2348080.8508988000887542TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG296850.062.6813743
CTCGTAT289950.061.86776742
CGTATGC304800.061.24146744
TATGCCG304600.061.1521746
ATCACGA306550.060.3277934
TCTCGTA297300.059.3728341
ATGCCGT314150.059.3033547
TCACGAT306550.059.0149335
GTATGCC320250.058.27349545
ATCTCGT301000.058.0847240
ACGATCT302600.057.6827637
CATCACG334300.055.9902633
CACGATC314500.055.58859336
CGTCTGA345600.055.40088316
ACACGTC348900.055.12649513
ATCGGAA356700.055.0350072
ACGTCTG351200.054.41753415
GATCGGA366850.053.2164571
CCGTCTT352100.052.71253250
CACACGT367650.052.50129312
Produced by FastQC (version 0.11.5)