FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23352230
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1115330.47761177412178624TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG154150.062.53365743
CGTATGC154700.062.3788144
CTCGTAT137250.061.71607642
TCTCGTA137100.061.65594541
ATGCCGT154650.061.28576747
TATGCCG160250.060.1056146
TGCCGTC156450.059.9987548
AATCTCG144650.059.6716539
GCCGTCT152650.059.0618649
ATCTCGT148250.058.12830740
CGTCTGA191150.058.12217316
CCGTCTT159250.057.49318350
ACACGTC197600.056.34667613
ACGTCTG198100.056.241615
CGTCTTC159750.055.2098851
GATCGGA208050.054.1099431
ATCGGAA209500.054.0102272
GTCGTAT26200.053.03767442
CACGTCT212200.052.61898414
GTATGCC188000.051.73684345
Produced by FastQC (version 0.11.5)