FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25007881
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC681730.2726060636644904TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA18250.057.53851741
TCGTATG95600.057.48277343
TCTCGTA97250.057.44385541
CGTATGC103850.057.29728344
GATCGGA186150.057.0148771
CTCGTAT98200.056.95942742
ATCGGAA188350.056.6190832
CGCGTAT18500.056.5717742
ACACGTC186500.056.35883713
CGTCTGA186900.055.97827516
ATGCCGT102900.055.2366747
TATGCCG110000.054.66277746
ACGTCTG192250.054.511215
ATCGCGT27300.053.97815740
ATCTCGT121050.052.6844140
CACGTCT201900.052.06118814
TCGGAAG206650.051.7743073
CATCTCG128650.051.61253739
TGCCGTC111350.051.107648
CGGAAGA209550.050.9909864
Produced by FastQC (version 0.11.5)