FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28661311
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37450.031.21671543
CTCGTAT38450.030.40488842
CGTATGC38400.030.35295544
TCTCGTA39250.029.78517241
TATCTCG41050.028.47907339
TATGCCG41100.028.10176746
CGCCAAT43150.027.57746333
ATGCCGT44450.026.0623747
ATCTCGT49100.023.80989640
ACGCCAA51850.023.62519832
GTCACGC56150.021.3796129
TGCCGTC55000.020.99946248
CCGTCTT57800.020.1032250
GCCGTCT62550.018.29686449
TCACGCC67750.017.8740230
CGTCTTC65650.017.3795151
AGTCACG72300.016.99120728
CACGCCA77450.016.08735831
CGTCTGA80050.015.34835216
GTATGCC79650.015.29180345
Produced by FastQC (version 0.11.5)