FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26670376
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC858440.32187022785130587TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA124250.058.7409671
TCTCGTA111500.058.10704441
CGTATGC117150.057.8429644
TCGTATG117750.057.6083243
CTCGTAT113700.057.32124742
ATCGGAA129200.056.778362
ATGCCGT120300.056.29631847
CGTCTGA127000.056.1971116
AATCTCG116200.055.3045139
TGCCGTC122550.055.11941548
TATGCCG124600.054.43818746
GCCGTCT123250.054.32350549
CCGTCTT124000.054.22074550
ACGTCTG132800.053.84774415
ACACGTC134250.053.70756513
ATCTCGT122400.052.760840
TCGGAAG143350.051.4666823
CGTCTTC130950.050.67477451
CGGAAGA145200.050.4493874
CACGTCT150900.047.66650414
Produced by FastQC (version 0.11.5)