FastQCFastQC Report
Thu 13 Dec 2018
HGHCCBGX9_n01_nk05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHCCBGX9_n01_nk05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27868072
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1193690.42833605424874743TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGTA226750.061.53950534
CACCGAT230750.060.83708231
TCTCGTA163250.060.67820741
ACCGATG231500.060.5341632
CTCGTAT163550.060.3956942
CGTATGC169000.059.77248444
CCGATGT233250.059.76488533
TATCTCG179850.059.65081839
TCGTATG159300.059.4798243
ATGCCGT166300.058.67597247
TCGCGTA20300.058.45250741
GTCACCG241150.058.1987629
TATGCCG177600.057.52454846
TATCGCG29650.057.49110439
TCACCGA244500.057.47293530
CGTCTGA248100.057.322916
TGCCGTC168500.056.954348
ATCTCGT187600.056.1793440
ACACGTC257150.055.6843513
ACGTCTG256450.055.51086415
Produced by FastQC (version 0.11.5)