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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2018-12-07, 19:12 based on data in: /beegfs/mk5636/logs/html/HGHC5BGX9/merged


        General Statistics

        Showing 34/34 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        HGHC5BGX9_n01_AHm01
        45.1%
        49%
        29.1
        HGHC5BGX9_n01_AHm02
        39.5%
        49%
        17.5
        HGHC5BGX9_n01_AHm03
        46.5%
        48%
        15.5
        HGHC5BGX9_n01_AHm04
        55.6%
        48%
        27.5
        HGHC5BGX9_n01_AHm05
        43.5%
        49%
        29.5
        HGHC5BGX9_n01_AHm06
        45.8%
        49%
        28.8
        HGHC5BGX9_n01_AHm07
        40.2%
        49%
        19.9
        HGHC5BGX9_n01_AHm08
        38.5%
        49%
        17.3
        HGHC5BGX9_n01_AHm09
        41.4%
        49%
        21.2
        HGHC5BGX9_n01_AHm10
        46.1%
        49%
        28.0
        HGHC5BGX9_n01_AHm11
        52.3%
        48%
        30.2
        HGHC5BGX9_n01_AHm12
        45.9%
        48%
        14.3
        HGHC5BGX9_n01_AHm13
        54.6%
        47%
        11.9
        HGHC5BGX9_n01_AHm14
        59.9%
        47%
        18.4
        HGHC5BGX9_n01_AHm15
        36.8%
        49%
        13.8
        HGHC5BGX9_n01_AHm16
        37.7%
        49%
        15.4
        HGHC5BGX9_n01_undetermined
        68.5%
        52%
        20.5
        HGHC5BGX9_n02_AHm01
        43.9%
        49%
        29.1
        HGHC5BGX9_n02_AHm02
        37.5%
        49%
        17.5
        HGHC5BGX9_n02_AHm03
        45.5%
        48%
        15.5
        HGHC5BGX9_n02_AHm04
        54.0%
        48%
        27.5
        HGHC5BGX9_n02_AHm05
        42.4%
        49%
        29.5
        HGHC5BGX9_n02_AHm06
        44.7%
        49%
        28.8
        HGHC5BGX9_n02_AHm07
        39.1%
        49%
        19.9
        HGHC5BGX9_n02_AHm08
        37.2%
        49%
        17.3
        HGHC5BGX9_n02_AHm09
        40.0%
        49%
        21.2
        HGHC5BGX9_n02_AHm10
        44.5%
        49%
        28.0
        HGHC5BGX9_n02_AHm11
        50.9%
        48%
        30.2
        HGHC5BGX9_n02_AHm12
        44.6%
        48%
        14.3
        HGHC5BGX9_n02_AHm13
        52.7%
        47%
        11.9
        HGHC5BGX9_n02_AHm14
        57.4%
        47%
        18.4
        HGHC5BGX9_n02_AHm15
        35.3%
        49%
        13.8
        HGHC5BGX9_n02_AHm16
        36.6%
        49%
        15.4
        HGHC5BGX9_n02_undetermined
        66.6%
        52%
        20.5

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 17/17 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        20,475,865
        5.7
        AHm01
        29,092,494
        8.1
        AHm02
        17,517,106
        4.9
        AHm03
        15,539,219
        4.3
        AHm04
        27,486,188
        7.7
        AHm05
        29,457,885
        8.2
        AHm06
        28,757,614
        8.0
        AHm07
        19,874,169
        5.5
        AHm08
        17,296,403
        4.8
        AHm09
        21,167,380
        5.9
        AHm10
        28,018,492
        7.8
        AHm11
        30,213,640
        8.4
        AHm12
        14,301,050
        4.0
        AHm13
        11,878,160
        3.3
        AHm14
        18,362,199
        5.1
        AHm15
        13,823,845
        3.9
        AHm16
        15,388,156
        4.3

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        388,379,544
        358,649,865
        5.7
        2.8

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (101bp).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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