FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20475865
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16699448.155670102337558No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN329830.16108232790165397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA9305.456968E-1210.7559459
CTACCGT11400.010.4431838
CGGGTAT9004.3783075E-99.47651
ACTACCG14100.08.768987
TCCGATC11403.583409E-108.7283541
CTCGAAT17500.08.6642281
CGGGGAT23700.08.5968241
CGGGGTT30800.08.461161
CTCGGAT17600.08.345781
CGGATCG5850.00134010358.1062373
CGACGAT11302.4754627E-87.96696851
CGGACAT16101.2732926E-117.6518321
CGGGCAT26700.07.6308891
TCGTGTA57100.07.601490514-15
CTGGGAT82100.07.50270941
CCGATCT15302.4920155E-107.43842032
CGACGGA7703.2267344E-47.3842851
CTTGGAT47250.07.22018961
CGAGGAT23000.07.2103791
CTGGAAT63150.07.2030391
Produced by FastQC (version 0.11.5)