FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15388156
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA15800.012.6280259
CTACCGT20450.011.6138118
CTCGGAT42850.09.8612611
GCCGTAT19350.09.70217560-61
CTCGGTT28600.09.6284261
CTCGTAT16300.09.3208541
CGTATCA19450.09.16261562-63
CCGGGAT44300.08.7882711
CGGGGTT50900.08.7680581
CGCCGTA25950.08.42524758-59
CGACCGT12703.3105607E-108.2245531
TCGCGTT10200.08.1466830-31
CGCGGGT28800.08.0778711
ACTACCG27700.08.0576327
CGACAAC29500.08.0471361
CGGGGAT45150.07.99188331
CGGGTAT17250.07.98182541
CGGCGAT28000.07.96953871
CGAAGAT57500.07.9267791
CTCGGGT42450.07.7172891
Produced by FastQC (version 0.11.5)