FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13823845
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA15350.016.4021439
CGTATCA18850.015.0004662-63
GCCGTAT22050.013.14837660-61
CTACCGT20050.013.0301678
CGCCGTA25850.011.67525658-59
CGGGGAT41600.010.95731
TACGTCC30250.010.28291332-33
GTACGTC29950.010.2273532-33
TGGTCGC31900.010.20599854-55
CCGTATC29850.010.190289560-61
TCGCCGT29100.09.63634358-59
CGCGGGT25250.09.5903491
ACTACCG24100.09.4588057
GTGTACG32550.09.19007630-31
CGGGTAT16300.09.0302491
ACCGTAT15400.08.48512610-11
CCGGGAT39250.08.4680611
CGACCGT11403.1686795E-98.3301111
TGTACGT39850.08.1619130-31
CGGGGTT48900.08.0592551
Produced by FastQC (version 0.11.5)