FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18362199
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCT246780.13439566796983302No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC230900.1257474663029194Illumina Single End PCR Primer 1 (96% over 33bp)
CAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAGTATCATTGGGATCT191590.10433935499773203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT43150.029.62926160-61
CGTATCA43450.029.2031362-63
TCGCCGT45700.026.83264558-59
CGCCGTA53000.026.1858758-59
TGGTCGC53800.025.79645354-55
TACCGTA21600.025.732019
GACATAA142650.023.2316655
CTACCGT25750.021.9519678
GTCGCCG63250.021.8675856-57
TAATGGA160600.020.26229
TCGGTGG70950.019.9274650-51
TCTATGC46900.019.7499288
CCGTATC71400.019.70347860-61
AGACATA179900.019.2905164
GTAGATC66150.018.56921844-45
CGGTGGT68950.018.40424752-53
ACTACCG30800.018.040227
CTCGGTG65750.017.88944450-51
ATAATGG185950.017.4473028
ACATAAT194800.017.1360826
Produced by FastQC (version 0.11.5)