FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878160
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCT150590.12677889504771783No Hit
CAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAGTATCATTGGGATCT120550.10148878277443645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA18200.026.63445362-63
GCCGTAT18850.025.84502260-61
GACATAA88100.022.9578695
TACCGTA12250.022.105399
TCGCCGT22450.021.17181658-59
CGACCGT8150.020.9716991
TCTATGC32800.020.2755838
CGCCGTA26800.019.86358858-59
TAATGGA104000.019.505369
AGACATA111750.019.4140134
TGGTCGC27900.019.16537554-55
CTACCGT15550.018.6345398
CGATCGA23200.017.8023744
CGCAAAA29100.017.4573971
ACTCTAT41450.017.2983686
CTATGCT40250.016.9963659
ATAATGG116200.016.9652848
ACATAAT122150.016.6380736
CATAATG126550.016.6251537
GTCGCCG31950.016.51333656-57
Produced by FastQC (version 0.11.5)