FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14301050
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA13900.015.3793389
TAATGGA92150.014.8469449
AACATAA124350.012.6770455
ACATAAT115300.012.0668226
CTACCGT18700.011.6847988
TATTCGC47200.011.47860160-61
ATAATGG122350.011.2201558
CATAATG129950.011.219727
ATATTCG47850.010.92538760-61
CAAAAAC178750.010.7051391
CGCAAAA33400.010.6622321
CGCGGGT22950.010.5516611
TCGCAAG49850.010.53137164-65
TTCGCAA52450.010.46470362-63
ATTCGCA51250.010.38519262-63
AAACATA156050.010.3138834
CGGGGAT35550.010.0173991
CCCGTAT19850.09.8074631
ACTACCG21950.09.7363887
CGCGGAT13200.09.7123231
Produced by FastQC (version 0.11.5)