FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30213640
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA28400.019.7375139
CTACCGT39050.014.9610168
TAATGGA201500.013.7678639
AACATAA260700.012.2384025
ACTACCG47300.011.9474867
ACATAAT244700.011.8560216
CGCAAAA66800.011.4433871
TATTCGC103200.010.66147660-61
ATATTCG99250.010.55903260-61
CGACCTA12650.010.5083514
CAAAAAC380500.010.2570461
ATAATGG271300.010.2069448
CCGTATG33600.010.18210-11
TTCGCAA110700.010.15257762-63
CGTATCA30900.010.15028662-63
CGTATGG28350.010.14361212-13
GCAAAAG275050.010.132851
CATAATG286950.010.0786367
AAACATA339600.09.9815414
CGCGGGT44250.09.6568261
Produced by FastQC (version 0.11.5)