FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28018492
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA28500.014.6680489
CGTATCA37450.012.1821962-63
CTACCGT37650.012.1116138
GCCGTAT39900.011.85217660-61
CGCCGTA47950.011.54756158-59
CGCGGGT52000.010.7740711
CGCGTAA7001.0671811E-69.49579051
CGGGGTT89550.09.331431
ACTACCG48300.09.2422397
CTCGTAT28150.09.1078621
CGGGGAT84150.08.8582231
CTCGGTT53300.08.7297131
CCGTATC62400.08.49255160-61
CTCGGAT78950.08.419321
TCGCCGT59500.08.227658-59
CGCGAAT14801.4551915E-118.0200931
CGCGGAT37450.07.98711351
CGGGATT46300.07.7935221
TGGTCGC65350.07.74563354-55
CGACCGT22200.07.699291
Produced by FastQC (version 0.11.5)