FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21167380
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT32550.015.40503860-61
CGTATCA31300.015.10752662-63
CGCCGTA36700.014.2461458-59
TACCGTA21200.012.3243729
CCGTATC46800.011.01907760-61
TCGCCGT45950.010.80939458-59
TGGTCGC48900.010.25415354-55
CTACCGT26050.010.0287958
CGCTAAT11807.2759576E-118.8516461
GTCGATT16200.08.792041
CTCGGTT42200.08.6628681
CGGGAAT48850.08.5526661
CGGGGTT70900.08.5043461
CGATAAC23050.08.4449351
GTCGCCG64850.08.13553556-57
CTCGGAT59800.08.0980861
CGGGGAT63200.08.0380391
CTATAGT71850.07.90023636-37
TAGGCTA74250.07.899164732-33
GGCTATA74650.07.889719534-35
Produced by FastQC (version 0.11.5)