FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17296403
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA16900.012.9301459
CGCGGGT34700.010.6725341
CGGGTAT20250.010.3164441
CGGGGTT59750.010.0123291
CTACCGT23300.09.9889178
GTCGATT12700.09.3462781
CGGGGAT51350.08.9687871
CGCGGAT20300.08.8877131
CGGGAAT41200.08.7582791
CTCGGAT45900.08.5855451
CTGGAAT176050.08.3334511
CCGCAAT25150.08.3064821
CGTATCG16650.08.2688193
CGCACAT33300.08.1270521
TCGCGTT13150.07.94414430-31
CGGGATT27600.07.91318271
TCGGATT30200.07.86043642
CCCGTAT24950.07.8021761
GATCGGA199600.07.74214994-95
GGGGAAT88350.07.7385281
Produced by FastQC (version 0.11.5)