FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19874169
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA27650.014.00716762-63
GCCGTAT30850.012.78682460-61
CGCCGTA35800.012.74486158-59
CCGTATC42700.010.29564860-61
CGGGGAT59150.010.194121
TACCGTA18650.010.1884789
CGGGAAT45600.09.787321
TGGTCGC46850.09.58692954-55
TACGTCC44550.09.43424932-33
TCGCCGT44100.09.21455758-59
CGGGGTT68650.09.060071
CGCGGGT38600.08.7331691
TCGGATT34000.08.6573532
CTACCGT24800.08.6188138
GTACGTC45200.08.56309832-33
GGGGAAT105550.08.5466551
CGCGTAT5000.0029192238.5462051
CTCGGAT55700.08.4388021
CTTGGAT143500.08.4039341
GTGTACG49350.08.1785930-31
Produced by FastQC (version 0.11.5)