FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28757614
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA28800.017.8153849
CTACCGT33750.015.7639518
ACTACCG48900.011.2658957
CGCGAAT14900.010.8339311
CGGGGAT86400.010.4407871
CGTATCA39000.09.80983962-63
GCCGTAT41500.09.678061560-61
CGCCGTA47550.09.39646758-59
CGCGGGT56300.09.2763611
CCCGTAT45550.09.0682671
CTCGGAT79550.09.0121861
CGTATGG30350.08.53577312-13
CGGGGTT94600.08.3312541
CCGTATG35100.08.25823310-11
CTCGAAT63950.08.0181961
CTCGTAT31200.07.91301871
CTCGGTT56750.07.86421541
TCGGATT49450.07.68013432
CGCGGTT35850.07.5488211
CTGGAAT292200.07.5068061
Produced by FastQC (version 0.11.5)