FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29457885
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA28450.014.5268459
CTACCGT39000.011.6922318
CGGGGAT85350.09.8458851
CTCGTAT28550.09.6451031
ACTACCG48850.09.5270387
CTCGGAT81400.09.2154741
CGGGGTT101400.08.5215481
CGCGTAT6807.462509E-58.3783281
CGCGGGT53950.08.360211
CGGGTAT34000.08.2386891
CTGGAAT299100.07.7303221
CCGGGAT82300.07.7301871
GATCGGA363150.07.62800194-95
CCGTATG35100.07.58091910-11
CTCGGTT53850.07.49407861
GGGGAAT153850.07.4371161
CGTATGG31400.07.417405612-13
ATCGGAA345650.07.327464694-95
TCGGATT48750.07.30362562
CGACCTA15101.4442776E-97.2310364
Produced by FastQC (version 0.11.5)