FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27486188
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA29000.020.804149
GACATAA166550.019.6121965
CTACCGT34900.018.2381138
GCCGTAT39900.016.9151860-61
CGTATCA36350.016.53847562-63
AGACATA214650.016.2115554
CGACCGT19900.015.7464531
TAATGGA208800.015.1981259
ACTACCG43550.014.8304157
CGCCGTA52050.013.74315558-59
ATAATGG232050.013.5921168
CCGTGTA56550.013.35679540-41
CATAATG243550.013.2983827
ACATAAT249150.012.8830776
CGATCGA47000.012.322924
CGCGTAT5152.1365668E-711.9847241
TCGCCGT54750.011.7632158-59
TGGTCGC60950.011.502727554-55
CCGTATG32250.011.41899610-11
ACGATCG66050.011.2121363
Produced by FastQC (version 0.11.5)