FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539219
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAA88350.018.0567045
TACCGTA14500.016.0532579
AGACATA118900.014.1746764
TAATGGA112200.013.8025659
GCCGTAT18050.013.5600660-61
CGACCGT10200.013.0335341
CGATCGA25900.013.0144314
ATAATGG121800.012.8305688
CATAATG127350.012.4176417
CTACCGT19450.012.21098
ACATAAT131950.011.9470216
CGTATCA19650.011.60849762-63
CGCCGTA23700.011.0294858-59
ACGATCG37800.010.8009543
CGAGAAT44150.010.6465481
CGATTCA50450.010.5395844
ACTACCG25800.010.3077087
CAACGAT84150.010.2688461
GATCGAA52950.010.2222065
CGCGGGT30950.010.1248271
Produced by FastQC (version 0.11.5)