FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17517106
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA15750.016.8901549
CTACCGT22650.010.9052098
CTCGTAT18150.09.417891
ACTACCG30300.09.4038257
CTCGGAT47700.08.9588411
CTTGGAT121850.08.8066471
CGGGATT29900.08.7341211
CGGGGAT52650.08.65766051
CCGGGAT46350.08.6051261
CGTATCA19350.08.47289462-63
CGCGGGT32900.08.226341
CGCGAGT15650.08.1917581
CGCGAAT8903.1319541E-68.0025611
CTGGAAT169950.07.878721
CGCTAAT10901.1012162E-77.8410411
CTCGAAT37600.07.82945541
CCGTATG19100.07.711829710-11
TCGGATT26600.07.67506932
ATCGGAA197650.07.63846294-95
CGGGAAT42900.07.6369421
Produced by FastQC (version 0.11.5)