FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n02_AHm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n02_AHm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29092494
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA28650.014.4255079
CGTATCA35750.010.70119362-63
CTACCGT39050.010.2176058
CGCCGTA43750.09.94052358-59
CGCGGGT54050.09.8381751
CGGGGAT85350.09.7904211
GCCGTAT40550.09.72849360-61
CGGGGTT97600.09.3885761
CTCGGAT79300.09.0405731
ACTACCG50050.08.3498877
CGGGAAT65050.08.0285591
CGGGATT49000.07.7515071
TCGGATT49550.07.56916572
TGGTCGC61150.07.461844454-55
CGCGTAT7009.306658E-47.46082541
CCGTATC57100.07.449811560-61
CTGGAAT290800.07.4449651
CCGGGAT79800.07.43702651
CGGGCTT89650.07.4142971
TCGCCGT52900.07.27772358-59
Produced by FastQC (version 0.11.5)