FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20475865
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18442089.006740374582465No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208580.10186627036269287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG32250.027.48004334-35
CTCGTAT42850.022.81507344-45
TCTCGTA45300.022.3126842-43
AATGCGC40150.022.13202934-35
ATCGGAA156250.021.787782
AGAGCAC155100.021.5870138
GATCGGA154850.021.4431721
TCGGAAG157500.021.4337673
CACGTCT90400.021.2703214-15
CGGAAGA162800.020.9683554
CGTCTGA95350.020.41705716-17
CACACGT95700.020.36260212-13
ACGTCTG85000.019.61652814-15
TGCGCAT44950.019.40628836-37
ATGCCGT55100.019.4027948-49
ATGCGCA46600.019.0243636-37
ACACGTC87600.018.89783112-13
ATCTCGT52300.018.64577542-43
GCGCATC44700.017.40707638-39
CTAATGC53100.016.28637732-33
Produced by FastQC (version 0.11.5)