FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15388156
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT372010.24175086345628416TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG70550.036.65995434-35
CGTATGC60050.035.9901646-47
TATGCCG62200.035.5097848-49
CTCGTAT60800.033.9445644-45
TCGTATG56800.033.8262844-45
AATGCGC81250.029.81520834-35
TCTCGTA72800.026.68549242-43
ATCTCGT79550.026.63041742-43
ATGCGCA97150.026.62207436-37
GCGCATC93900.026.15215938-39
TGCGCAT94900.025.00092736-37
CGCGTAT7550.023.90675544-45
CTAATGC107150.022.96292532-33
ATGCCGT90100.022.87970248-49
GTATGCC92600.022.80059446-47
GTCACTA111650.022.24973528-29
ACTAATG120050.021.99896632-33
CCGTCTT98100.021.93390852-53
CGCATCT99550.021.5903638-39
ATCGGAA230100.021.1394732
Produced by FastQC (version 0.11.5)