FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13823845
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT589970.4267770652810416TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG107000.040.59634434-35
CGTATGC90300.040.26647646-47
TATGCCG91300.040.05941448-49
CTCGTAT90550.038.4766344-45
TCGTATG85650.038.23774344-45
AATGCGC116000.034.88739834-35
TCTCGTA97750.033.43159542-43
ATGCGCA130250.033.33136436-37
ATCTCGT106650.033.1580742-43
GCGCATC126550.032.14752238-39
TGCGCAT127600.030.98975836-37
CCGTCTT115300.029.49667452-53
CTAATGC138250.029.47859432-33
ACTAATG150550.029.13672632-33
GTATGCC119800.029.0030546-47
GAGCACA285600.028.9214259
AGAGCAC285000.028.8489868
ATCGGAA280750.028.8308872
ATGCCGT116600.028.8212348-49
CGCGTAT8750.028.77088544-45
Produced by FastQC (version 0.11.5)