FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18362199
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT2566161.397523248713294TruSeq Adapter, Index 3 (97% over 36bp)
CAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCT250800.1365849482406764No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTA195680.10656675706433635TruSeq Adapter, Index 3 (97% over 36bp)
CAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAGTATCATTGGGATCT193530.10539587333739275No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCGCGTAT185360.10094651517500708TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC328300.045.25661546-47
TATGCCG331750.045.15823748-49
CTCGTAT316300.044.86368644-45
TAATGCG396250.044.19648434-35
ATCTCGT328800.043.36751642-43
CGCGTAT25150.043.2497644-45
GCGCATC380650.043.09383438-39
ATGCGCA403850.042.88835536-37
GAGCACA767100.042.5017479
TCGTATG314400.041.88661644-45
CCGTCTT337950.041.68752352-53
AGAGCAC798750.040.8949478
TCTCGTA319850.040.8609842-43
AATGCGC399950.040.2603834-35
TGCCGTC358900.039.95532650-51
CGGAAGA817150.039.9045684
TGCGCAT394450.039.68976236-37
GCCGTCT323700.039.44314650-51
TCGGAAG831450.039.230643
AAGAGCA835750.039.186877
Produced by FastQC (version 0.11.5)