FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11878160
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1094680.9215905493780182TruSeq Adapter, Index 3 (97% over 36bp)
CAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCT152460.12835321295554195No Hit
CAACGATTCAAGTGACCCGCTTGTTGTTGCCGCGAGTATCATTGGGATCT123290.10379553735595413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC145900.044.56880646-47
CTCGTAT138350.044.51181444-45
TATGCCG147600.044.4899248-49
TAATGCG175250.044.0570534-35
ATCTCGT146250.042.35099842-43
TCGTATG137350.042.10389344-45
GCGCATC172300.041.8473138-39
ATGCGCA185050.041.27448336-37
CGCGTAT13450.040.61229344-45
TCTCGTA142800.040.2641542-43
GAGCACA365300.039.8789949
AATGCGC180050.039.7827434-35
CCGTCTT154000.039.5572352-53
CGGAAGA373900.038.7716834
TGCGCAT179600.038.77147336-37
AGAGCAC380650.038.370678
TCGGAAG381650.038.1094633
GCCGTCT147550.037.8415650-51
TGCCGTC166800.037.8170550-51
CATCTCG157100.037.58165740-41
Produced by FastQC (version 0.11.5)