FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14301050
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT292870.20478915883798743TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC46550.036.4784746-47
TAATGCG55100.036.46471434-35
TATGCCG46800.036.08068548-49
TCGTATG44650.034.94579344-45
CTCGTAT48050.034.25238844-45
AATGCGC63200.030.2505234-35
ATCTCGT58650.028.58825942-43
TCTCGTA56050.027.75343142-43
ATGCGCA75800.026.53795636-37
GCGCATC72650.026.21745338-39
TGCGCAT74000.025.25784536-37
CGCGTAT4500.024.80483644-45
CTAATGC82650.023.18906432-33
GTATGCC74150.022.42007846-47
CATCTCG71950.022.37951340-41
ATGCCGT71550.022.23904648-49
CCGTCTT74850.021.73473452-53
GCCGTCT71000.021.30755450-51
ACTAATG98000.020.9382432-33
CGCATCT80000.020.60256238-39
Produced by FastQC (version 0.11.5)