FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30213640
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT756610.25042000897607836TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG116500.037.97860748-49
CGTATGC115400.037.8055446-47
TAATGCG137400.037.5603334-35
TCGTATG107650.036.1368744-45
CTCGTAT118400.035.56376344-45
AATGCGC149100.032.30328834-35
ATCTCGT140050.030.52390142-43
TCTCGTA130100.030.1931842-43
ATGCGCA172600.029.69366636-37
GCGCATC169100.028.9175838-39
TGCGCAT171450.027.48256936-37
CGCGTAT12300.025.68012844-45
CTAATGC193050.025.14587632-33
GTATGCC168300.024.86416646-47
CCGTCTT166500.024.83352752-53
ATGCCGT168500.023.97488448-49
GCCGTCT161100.023.39554250-51
CATCTCG176050.023.1353840-41
ACTAATG226250.023.11449432-33
CGCATCT186250.022.78649338-39
Produced by FastQC (version 0.11.5)