FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28018492
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1047910.37400656680595085TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG181950.039.0017534-35
TATGCCG156450.038.96769348-49
CGTATGC156900.038.34138546-47
CTCGTAT155450.037.1100544-45
TCGTATG145300.036.809344-45
AATGCGC199950.033.1270434-35
TCTCGTA173100.031.08977742-43
ATGCGCA231150.030.41247736-37
ATCTCGT191600.030.21989442-43
GCGCATC220950.030.07503538-39
TGCGCAT221400.029.12367636-37
CGCGTAT16400.027.80431244-45
GTATGCC215050.027.0019446-47
CTAATGC247400.026.90782432-33
ACTAATG267450.026.83533532-33
GTCACTA253000.026.5186828-29
ATGCCGT213050.026.34125948-49
CCGTCTT219550.026.25373652-53
TCACTAA285850.025.30733930-31
CGCATCT228650.025.0840938-39
Produced by FastQC (version 0.11.5)