FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21167380
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT750600.35460222285422194TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG131150.039.44088734-35
CGTATGC112450.038.94533546-47
TATGCCG114000.038.6867848-49
CTCGTAT111000.037.35745644-45
TCGTATG104950.035.8450244-45
AATGCGC145150.032.2170534-35
ATGCGCA168200.030.66816936-37
TCTCGTA123850.030.6625342-43
ATCTCGT139150.030.49979642-43
GCGCATC162300.030.14398438-39
TGCGCAT162800.028.11132636-37
CGCGTAT12550.027.8182444-45
ACTAATG200350.026.29240432-33
CCGTCTT159650.026.10756352-53
ATGCCGT153000.025.8606348-49
GTATGCC156850.025.7860146-47
CTAATGC182750.025.71855432-33
GTCACTA188050.025.13241828-29
TCACTAA215950.024.70094930-31
CGCATCT169700.024.01526538-39
Produced by FastQC (version 0.11.5)