FastQCFastQC Report
Fri 7 Dec 2018
HGHC5BGX9_n01_AHm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGHC5BGX9_n01_AHm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17296403
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT203250.11750998169966322TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41050.028.75413948-49
CGTATGC41050.028.63855646-47
TCGTATG40600.027.02557844-45
CTCGTAT44050.026.14898744-45
TATCTCG48200.021.72938540-41
TCTCGTA56500.018.70555542-43
ATCTCGT64000.018.03496642-43
ATGCCGT73950.014.9659748-49
GCTATCT72300.014.7818138-39
GTATGCC78750.014.11413746-47
CCGTCTT85700.013.99491452-53
AGCTATC84600.013.75558738-39
GCCGTCT82650.013.44803650-51
CTATCTC94900.012.73818340-41
TGCCGTC99550.012.38174950-51
CACGTCT110150.011.3842414-15
GAAGCTA110600.011.27348436-37
ACGTCTG101350.011.13075214-15
CGTCTGA114950.010.95015516-17
TACCGTA17900.010.8796019
Produced by FastQC (version 0.11.5)